Anticorps Polyclonal de lapin anti-ROCK2-Specific(C-term)

• Phare
• Validé par KD/KO

ROCK2-Specific(C-term) Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20248-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 20248-1-AP (same clone as 20248-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 20248-1-AP (same clone as 20248-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 20248-1-AP (same clone as 20248-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 20248-1-AP (same clone as 20248-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 20248-1-AP (same clone as 20248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 20248-1-AP (same clone as 20248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 20248-1-AP (same clone as 20248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 20248-1-AP (same clone as 20248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 20248-1-AP (same clone as 20248-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using ROCK2 antibody 20248-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20248-1-PBS cible ROCK2-Specific(C-term) dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Rho-associated, coiled-coil containing protein kinase 2
• Masse moléculaire calculée: 161 kDa
• Poids moléculaire observé: 164 kDa
• Numéro d’acquisition GenBank: NM_004850
• Symbole du gène: ROCK2
• Identification du gène (NCBI): 9475
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.

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