Anticorps Polyclonal de lapin anti-ROR1

ROR1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 20629-1-AP

Synonymes

NTRKR1, ROR1

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Galerie de données de validation

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  WB analysis of K-562 using 20629-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20629-1-AP (ROR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20629-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20629-1-AP (ROR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 20629-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20629-1-AP (ROR1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 20629-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20629-1-AP (ROR1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 20629-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20629-1-AP (ROR1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 20629-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20629-1-AP (ROR1 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

20629-1-AP cible ROR1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ROR1 Protéine recombinante Ag14586
• Nom complet: receptor tyrosine kinase-like orphan receptor 1
• Masse moléculaire calculée: 937 aa, 104 kDa
• Poids moléculaire observé: 130-140 kDa
• Numéro d’acquisition GenBank: BC006374
• Symbole du gène: ROR1
• Identification du gène (NCBI): 4919
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ROR1 is a member of the RTK family of orphan receptors related to muscle specific kinase and Trk neurotrophin receptors (PMID: 18546292). ROR1 is primarily expressed by neural crest cells during embryogenesis. High expression of ROR1 is reported in many types of malignancies and is thought to be involved in tumor growth, apoptosis, and epithelial-mesenchymal transition (PMID: 26245996). The human ROR1 gene encodes a 937-amino acid protein with an Ig-like domain, a cysteine-rich domain, a kringle domain, a tyrosine kinase domain and a proline-rich domain. The calculated molecular weight of ROR1 is 104 kDa, but ROR1 has multiple N-glycosylation sites that generate post-translationally modified ROR1 at 130 kDa (PMID: 24752542).

Publications

Species	Application	Title
human	WB	Oncol Rep; Wnt5a/ROR1 activates DAAM1 and promotes the migration in osteosarcoma cells.; Authors - Bin Dai
human	IHC	Virchows Arch; Expression of ROR1 has prognostic significance in triple negative breast cancer.; Authors - Hui-Ping Chien
human	IHC	Biomedicines; ROR1 Is Expressed in Diffuse Large B-Cell Lymphoma (DLBCL) and a Small Molecule Inhibitor of ROR1 (KAN0441571C) Induced Apoptosis of Lymphoma Cells.; Authors - Amineh Ghaderi
human	WB	Cancer Manag Res; Wnt5a induces ROR1 and ROR2 to activate RhoA in esophageal squamous cell carcinoma cells.; Authors - Xuping Wu
rat	IF	Am J Physiol Cell Physiol; Periodontal Ligaments Enhance Neurite Outgrowth in Trigeminal Ganglion Neurons through Wnt5a Production Induced by Mechanical Stimulation; Authors - Kaori Takahashi
human	IHC	Pharmaceutics; A Small Molecule Targeting the Intracellular Tyrosine Kinase Domain of ROR1 (KAN0441571C) Induced Significant Apoptosis of Non-Small Cell Lung Cancer (NSCLC) Cells; Authors - Amineh Ghaderi

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