Anticorps Polyclonal de lapin anti-ROR2

• Phare
• Validé par KD/KO

ROR2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 25842-1-AP

Synonymes

BDB, BDB1, EC:2.7.10.1, Neurotrophic tyrosine kinase, receptor-related 2, NTRKR2

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Galerie de données de validation

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  WB analysis using 25842-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25842-1-AP (ROR2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25842-1-AP

  WB result of ROR2 antibody (25842-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROR2 transfected HEK-293 cells.

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  WB analysis using 25842-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25842-1-AP (ROR2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules K-562, cellules T-47D

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

25842-1-AP cible ROR2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ROR2 Protéine recombinante Ag22959
• Nom complet: receptor tyrosine kinase-like orphan receptor 2
• Masse moléculaire calculée: 943 aa, 105 kDa
• Poids moléculaire observé: 105 kDa
• Numéro d’acquisition GenBank: BC130522
• Symbole du gène: ROR2
• Identification du gène (NCBI): 4920
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tyrosine-protein kinase transmembrane receptor ROR2 belongs to the ROR-family of receptor tyrosine kinases (RTKs) (PMID: 12815588). ROR2 is a developmentally regulated protein that has significant expression and roles in a wide variety of tissues during early embryonic development. It was established that ROR2 plays a key role in skeletal and neural organogenesis, as well as in midgut development in the mouse, but then becomes repressed in adult tissues. It may act as a receptor for wnt ligand WNT5A which may result in the inhibition of WNT3A-mediated signaling (PMID: 25614331). Mutations in ROR2 gene are associated with autosomal recessive Robinow syndrome and autosomal dominant brachydactyly type B (PMID: 12815588).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Stephen (Verified Customer) (08-02-2022)

IP performed using in HeLa cells in RIPA buffer using 5ug of ror2 antibody per 1ug/ml of protein lysate. pulldown performed using magnetic protein A beads (NEB). western blot probed with another ror2 specific antibody raised in mouse ( MABS1152 )

• Applications: Immunoprecipitation
• Primary Antibody Dilution: 5ug of protein used for IP
• Cell Tissue Type: HeLa

ROR2 Antibody Immunoprecipitation validation (5ug of protein used for IP dilution) in HeLa (Cat no:25842-1-AP)

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