Anticorps Monoclonal anti-ROR2

ROR2 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

1A12C5

N° de cat : 67906-1-Ig

Synonymes

BDB, BDB1, NTRKR2, ROR2

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Galerie de données de validation

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  WB analysis using 67906-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67906-1-Ig (ROR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis using 67906-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67906-1-Ig (ROR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67906-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67906-1-Ig (ROR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules 4T1, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules MDA-MB-231, cellules NIH/3T3, cellules T-47D

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

67906-1-Ig cible ROR2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ROR2 Protéine recombinante Ag31197
• Nom complet: receptor tyrosine kinase-like orphan receptor 2
• Masse moléculaire calculée: 943 aa, 105 kDa
• Poids moléculaire observé: 105 kDa
• Numéro d’acquisition GenBank: BC130522
• Symbole du gène: ROR2
• Identification du gène (NCBI): 4920
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tyrosine-protein kinase transmembrane receptor ROR2 belongs to the ROR family of receptor tyrosine kinases (RTKs) (PMID: 12815588). ROR2 is a developmentally regulated protein that has significant expression and roles in a wide variety of tissues during early embryonic development. It was established that ROR2 plays a key role in skeletal and neural organogenesis, as well as in midgut development in the mouse, but then becomes repressed in adult tissues. It may act as a receptor for wnt ligand WNT5A which may result in the inhibition of WNT3A-mediated signaling (PMID: 25614331). Mutations in the ROR2 gene are associated with autosomal recessive Robinow syndrome and autosomal dominant brachydactyly type B (PMID: 12815588).

Publications

Species	Application	Title
mouse	WB	J Nanobiotechnology; A novel microglia-targeting strategy based on nanoparticle-mediated delivery of miR-26a-5p for long-lasting analgesia in chronic pain; Authors - Yitian Lu
	WB	bioRxiv; Two functional forms of the Meckel-Gruber syndrome protein TMEM67 generated by proteolytic cleavage by ADAMTS9 mediate Wnt signaling and ciliogenesis; Authors - Manu Ahmed

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