Anticorps Polyclonal de lapin anti-RORA

• Phare
• Validé par KD/KO

RORA Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 10616-1-PBS

Synonymes

NR1F1, Nuclear receptor ROR-alpha, Nuclear receptor RZR-alpha, RAR-related orphan receptor A, Retinoid-related orphan receptor-alpha

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Galerie de données de validation

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  WB analysis using 10616-1-AP (same clone as 10616-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10616-1-AP (RORA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10616-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 10616-1-AP (same clone as 10616-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10616-1-AP (RORA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10616-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 10616-1-AP (same clone as 10616-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10616-1-AP (RORA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10616-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10616-1-AP (same clone as 10616-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10616-1-AP (RORA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10616-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 10616-1-AP (same clone as 10616-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10616-1-AP (RORA antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10616-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10616-1-PBS cible RORA dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RORA Protéine recombinante Ag0944
• Nom complet: RAR-related orphan receptor A
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC008831
• Symbole du gène: RORA
• Identification du gène (NCBI): 6095
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RORA is a steroid hormone receptor and has been implicated in the pathology of circadian rhythms, bone growth, angiogenesis, development of cones, cellular metabolism and a mediator in the immune and lipid metabolism pathways [PMID:21998696]. It binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence, which binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPAR gamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM and ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1 [PMID:11053433,14570920].

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