Anticorps Recombinant de lapin anti-RORA

RORA Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230217G6

N° de cat : 82930-1-PBS

Synonymes

230217G6, NR1F1, Nuclear receptor ROR-alpha, Nuclear receptor RZR-alpha, RAR-related orphan receptor A

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Galerie de données de validation

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  WB analysis using 82930-1-RR (same clone as 82930-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82930-1-RR (RORA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82930-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 82930-1-PBS

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 82930-1-PBS (RORA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat cerebellum using 82930-1-RR (same clone as 82930-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 82930-1-RR (RORA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82930-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 82930-1-RR (same clone as 82930-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 82930-1-RR (RORA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82930-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 82930-1-RR (same clone as 82930-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 82930-1-RR (RORA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82930-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 82930-1-RR (same clone as 82930-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 82930-1-RR (RORA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82930-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 82930-1-PBS

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 82930-1-PBS (RORA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 82930-1-RR (same clone as 82930-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using RORA antibody (82930-1-RR, Clone: 230217G6 ) at dilution of 1:350 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82930-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82930-1-PBS

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RORA antibody (82930-1-PBS, Clone: 230217G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 82930-1-RR (same clone as 82930-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RORA antibody (82930-1-RR, Clone: 230217G6 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82930-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 82930-1-RR (same clone as 82930-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RORA antibody (82930-1-RR, Clone: 230217G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82930-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82930-1-RR (same clone as 82930-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug RORA Recombinant antibody (82930-1-RR, Clone:230217G6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82930-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82930-1-PBS

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RORA Recombinant antibody, PBS Only (82930-1-PBS, Clone:230217G6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IHC: tissu de cervelet de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82930-1-PBS cible RORA dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RORA Protéine recombinante Ag0944
• Nom complet: RAR-related orphan receptor A
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 60-66 kDa
• Numéro d’acquisition GenBank: BC008831
• Symbole du gène: RORA
• Identification du gène (NCBI): 6095
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RORA is a steroid hormone receptor and has been implicated in the pathology of circadian rhythms, bone growth, angiogenesis, development of cones, cellular metabolism and a mediator in the immune and lipid metabolism pathways [PMID:21998696]. It binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence, which binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPAR gamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM and ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1 [PMID:11053433,14570920].

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