Anticorps Recombinant de lapin anti-RORA

RORA Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

230217G6

N° de cat : 82930-1-RR

Synonymes

230217G6, NR1F1, Nuclear receptor ROR-alpha, Nuclear receptor RZR-alpha, RAR-related orphan receptor A

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Galerie de données de validation

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  WB analysis using 82930-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82930-1-RR (RORA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of rat cerebellum using 82930-1-RR

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 82930-1-RR (RORA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat cerebellum using 82930-1-RR

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 82930-1-RR (RORA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 82930-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 82930-1-RR (RORA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 82930-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 82930-1-RR (RORA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 82930-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using RORA antibody (82930-1-RR, Clone: 230217G6 ) at dilution of 1:350 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 82930-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RORA antibody (82930-1-RR, Clone: 230217G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 82930-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RORA antibody (82930-1-RR, Clone: 230217G6 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 82930-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug RORA Recombinant antibody (82930-1-RR, Clone:230217G6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HepG2, cellules HuH-7, cellules L02, cellules PC-3
• Résultats positifs en IHC: tissu de cervelet de rat, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de rat,
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:175-1:700
• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

82930-1-RR cible RORA dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RORA Protéine recombinante Ag0944
• Nom complet: RAR-related orphan receptor A
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 60-66 kDa
• Numéro d’acquisition GenBank: BC008831
• Symbole du gène: RORA
• Identification du gène (NCBI): 6095
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RORA is a steroid hormone receptor and has been implicated in the pathology of circadian rhythms, bone growth, angiogenesis, development of cones, cellular metabolism and a mediator in the immune and lipid metabolism pathways [PMID:21998696]. It binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence, which binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPAR gamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM and ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1 [PMID:11053433,14570920].

Publications

Species	Application	Title
	WB	BMC Genomics; Transcriptomic and proteomic analyses of sclera in lens-induced myopic guinea pigs; Authors - He-Yan Li

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