Anticorps Polyclonal de lapin anti-MST4

• Phare
• Validé par KD/KO

MST4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 10847-1-AP

Synonymes

MASK, MST 4, Mst3 and SOK1 related kinase, MST4, RP6 213H19.1, RP6-213H19.1, STE20 like kinase MST4, STK26

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Galerie de données de validation

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  WB analysis using 10847-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10847-1-AP (MST4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10847-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10847-1-AP (RP6-213H19.1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10847-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10847-1-AP (RP6-213H19.1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10847-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10847-1-AP (RP6-213H19.1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 10847-1-AP

  IP Result of anti-MST4 (IP:10847-1-AP, 4ug; Detection:10847-1-AP 1:500) with MCF-7 cells lysate 2400ug.

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  IHC staining of human colon cancer using 10847-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10847-1-AP (RP6-213H19.1 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of MCF-7 using 10847-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 10847-1-AP (MST4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HL-60, cellules Jurkat, cellules L02, cellules MCF-7
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

10847-1-AP cible MST4 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MST4 Protéine recombinante Ag1302
• Nom complet: serine/threonine protein kinase MST4
• Masse moléculaire calculée: 16 kDa, 46 kDa
• Poids moléculaire observé: 47-50 kDa
• Numéro d’acquisition GenBank: BC017213
• Symbole du gène: RP6-213H19.1
• Identification du gène (NCBI): 51765
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IF	J Cell Sci; CLIC4 is a cytokinetic cleavage furrow protein that regulates cortical cytoskeleton stability during cell division.; Authors - Eric Peterman ; KD Validated
human	WB,IHC,IF	Int J Oncol; MST4 promotes hepatocellular carcinoma epithelial-mesenchymal transition and metastasis via activation of the p-ERK pathway.; Authors - Zhen-Hai Lin ; KD Validated
mouse	WB,IF	Brain Res Bull; MST4 attenuates NLRP3 inflammasome-mediated neuroinflammation and affects the prognosis after intracerebral hemorrhage in mice.; Authors - Xiaodong Wu
human	IHC	Onco Targets Ther; MST4 Regulates Epithelial-Mesenchymal Transition of Choriocarcinoma by Mediating TGF-β1 Expression.; Authors - Hanxi Yu
mouse	WB,IF	Behav Neurol; MST4 Kinase Inhibitor Hesperadin Attenuates Autophagy and Behavioral Disorder via the MST4/AKT Pathway in Intracerebral Hemorrhage Mice.; Authors - Xiaodong Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Guillaume (Verified Customer) (07-21-2022)

Antibody worked well, band present at 55kDa

• Applications: Western Blot
• Cell Tissue Type: Mice liver tumor/ Mice healthy liver

MST4 Antibody Western Blot validation ( dilution) in Mice liver tumor/ Mice healthy liver (Cat no:10847-1-AP)