Anticorps Monoclonal anti-RPA2

RPA2 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

1B9H11

N° de cat : 67999-1-Ig

Synonymes

REPA2, Replication factor A protein 2, replication protein A2, 32kDa, RF A protein 2, RP A p32, RP A p34, RPA2, RPA32, RPA34

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Galerie de données de validation

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  WB analysis using 67999-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of pig spleen using 67999-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 67999-1-Ig

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 67999-1-Ig

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit spleen using 67999-1-Ig

  rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 67999-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RPA2 antibody (67999-1-Ig, Clone: 1B9H11 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HeLa, cellules Jurkat, cellules MOLT-4, cellules THP-1, tissu splénique de lapin, tissu splénique de porc, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IF: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67999-1-Ig cible RPA2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RPA2 Protéine recombinante Ag24783
• Nom complet: replication protein A2, 32kDa
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC001630
• Symbole du gène: RPA2
• Identification du gène (NCBI): 6118
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Thr residues that are phosphorylated in sequential order in response to genotoxic stress.