Anticorps Polyclonal de lapin anti-RPAP3

RPAP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 23741-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HEK-293 using 23741-1-AP (same clone as 23741-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 23741-1-AP (same clone as 23741-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 23741-1-AP (same clone as 23741-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 23741-1-AP (same clone as 23741-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 23741-1-AP (same clone as 23741-1-PBS)

  IP result of anti-RPAP3 (IP:23741-1-AP, 4ug; Detection:23741-1-AP 1:500) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 23741-1-AP (same clone as 23741-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23741-1-AP (RPAP3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 23741-1-AP (same clone as 23741-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23741-1-AP (RPAP3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 23741-1-AP (same clone as 23741-1-PBS)

  Immunofluorescent analysis of HeLa cells using 23741-1-AP (RPAP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

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Informations sur le produit

23741-1-PBS cible RPAP3 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPAP3 Protéine recombinante Ag20661
• Nom complet: RNA polymerase II associated protein 3
• Masse moléculaire calculée: 76 kDa
• Poids moléculaire observé: 76 kDa
• Numéro d’acquisition GenBank: BC056415
• Symbole du gène: RPAP3
• Identification du gène (NCBI): 79657
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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