Anticorps Recombinant de lapin anti-RPE65

RPE65 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240518F1

Avis (1)

N° de cat : 83861-1-RR

Synonymes

Lutein isomerase, LCA2, EC:5.3.3.22, EC:3.1.1.64, All-trans-retinyl-palmitate hydrolase

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Galerie de données de validation

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  WB analysis using 83861-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83861-1-RR (RPE65 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules PC-3, cellules Y79

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83861-1-RR cible RPE65 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RPE65 Protéine recombinante Ag12356
• Nom complet: retinal pigment epithelium-specific protein 65kDa
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 65 kDa, 61 kDa
• Numéro d’acquisition GenBank: BC075036
• Symbole du gène: RPE65
• Identification du gène (NCBI): 6121
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RPE65(Retinal pigment epithelium-specific 65 kDa protein) is also named as retinoid isomerohydrolase with the calculated molecular weight og 61 kDa and belongs to the carotenoid oxygenase family. It is a microsomal protein with isomerase activity in the retinoid visual cycle that playing a role in the isomerisation of all-trans (vitamin A) to 11-cis retinal, the chromophore of the visual pigments. It also might be involved in retinoid uptake of keratinocytes.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alex (Verified Customer) (12-16-2024)

I have tested this RPE65 primary recombinant antibody used in western blotting, dilution factor 1 in 5000 as recommended in the website, used on ARPE-19 cells. I have clear bands with minimum non-specific bands at higher molecular weight, however, the clear bands correspond to ~95 kDa and not ~65 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: ARPE-19

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