Anticorps Polyclonal de lapin anti-RPGR

RPGR Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16891-1-PBS

Synonymes

RP3, X linked retinitis pigmentosa GTPase regulator, X-linked retinitis pigmentosa GTPase regulator, XLRP3

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Galerie de données de validation

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  WB analysis using 16891-1-AP (same clone as 16891-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

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  WB analysis using 16891-1-AP (same clone as 16891-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16891-1-AP (same clone as 16891-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 16891-1-AP (same clone as 16891-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

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  WB analysis of Y79 using 16891-1-AP (same clone as 16891-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 16891-1-AP (same clone as 16891-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 16891-1-AP (same clone as 16891-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 16891-1-AP (RPGR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 16891-1-AP (same clone as 16891-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 16891-1-AP (RPGR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 16891-1-AP (same clone as 16891-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16891-1-AP (RPGR Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16891-1-AP (same clone as 16891-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16891-1-AP (RPGR Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 16891-1-AP (same clone as 16891-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16891-1-AP (RPGR Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 16891-1-AP (same clone as 16891-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16891-1-AP (RPGR Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 cells using 16891-1-AP (same clone as 16891-1-PBS)

  IF result of anti-RPGR (16891-1-AP) in hTERT-RPE1 cell by Dr. Seongjin Seo. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16891-1-PBS cible RPGR dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPGR Protéine recombinante Ag10405
• Nom complet: retinitis pigmentosa GTPase regulator
• Masse moléculaire calculée: 113 kDa
• Poids moléculaire observé: 100-105 kDa, 70 kDa
• Numéro d’acquisition GenBank: BC031624
• Symbole du gène: RPGR
• Identification du gène (NCBI): 6103
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RPGR is a protein with a series of six RCC1-like domains (RLDs), characteristic of the highly conserved guanine nucleotide exchange factors. The encoded protein is found in the Golgi body and interacts with RPGRIP1. This protein localizes to the outer segment of rod photoreceptors and is essential for their viability. Mutations in this gene have been associated with X-linked retinitis pigmentosa (XLRP). Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length natures of only some have been determined. This antibody can recognize all the isoforms of RPGR.

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