Anticorps Polyclonal de lapin anti-RPH3A

RPH3A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11396-1-PBS

Synonymes

Rabphilin 3A, Rabphilin, KIAA0985, Exophilin-1, Exophilin1

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Galerie de données de validation

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  WB analysis of COLO 320 using 11396-1-AP (same clone as 11396-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11396-1-AP (RPH3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11396-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11396-1-AP (same clone as 11396-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11396-1-AP (RPH3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11396-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 11396-1-AP (same clone as 11396-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11396-1-AP (RPH3A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11396-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 11396-1-AP (same clone as 11396-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11396-1-AP (RPH3A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11396-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 11396-1-AP (same clone as 11396-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using RPH3A antibody (11396-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11396-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11396-1-PBS cible RPH3A dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPH3A Protéine recombinante Ag1956
• Nom complet: rabphilin 3A homolog (mouse)
• Masse moléculaire calculée: 690 aa, 76 kDa
• Poids moléculaire observé: 76 kDa
• Numéro d’acquisition GenBank: BC017259
• Symbole du gène: RPH3A
• Identification du gène (NCBI): 22895
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RAB3A is a small G protein that is thought to act in exocytosis of neurotransmitters and hormones in synaptic neurotransmission and cell-cell communication. RPH3A (RPH3A), a RAB3A effector, is a neuronal C2 domain tandem containing protein that is involved in the neuronal transmitter process. Huntington's disease (HD) is a hereditary neurodegenerative disorder characterized by progressive motor disturbances, cognitive defects and dementia. Level of rabphilin 3A is substantially decreased in synapses of most brain regions in R6/1 transgenic mouse model of HD suggesting a role of RPH3A in impaired synaptic transmission.

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