Anticorps Recombinant de lapin anti-RPL13

RPL13 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, singe, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250752D2

N° de cat : 86205-2-PBS

Synonymes

60S ribosomal protein L13, BBC1, D16S444E, Large ribosomal subunit protein eL13, OK/SW-cl.46

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Galerie de données de validation

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  WB analysis using 86205-2-RR (same clone as 86205-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86205-2-RR (RPL13 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86205-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86205-2-RR (same clone as 86205-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RPL13 antibody (86205-2-RR, Clone: 250752D2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86205-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86205-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86205-2-RR against Human RPL13 were performed. The affinity constant is 0.10 nM.

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Informations sur le produit

86205-2-PBS cible RPL13 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, singe, souris

• Réactivité: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RPL13 Protéine recombinante Ag1814
• Nom complet: ribosomal protein L13
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC014167
• Symbole du gène: RPL13
• Identification du gène (NCBI): 6137
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL13 may be a plausible candidate tumor suppressor gene, as knocking down L13 expression using small interfering RNA (siRNA) resulted in drastic attenuation of cancer cell growth with significant G1 and G2/M arrest of the cell cycle[PMID:16786168].

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