Anticorps Monoclonal anti-RPL17

RPL17 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C5E6

N° de cat : 67223-1-PBS

Synonymes

1C5E6, Large ribosomal subunit protein uL22, PD-1, uL22

---

Galerie de données de validation

• 

  WB analysis using 67223-1-Ig (same clone as 67223-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67223-1-Ig (RPL17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67223-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 67223-1-Ig (same clone as 67223-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67223-1-Ig (RPL17 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67223-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 67223-1-Ig (same clone as 67223-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67223-1-Ig (RPL17 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67223-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67223-1-Ig (same clone as 67223-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67223-1-Ig (RPL17 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67223-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67223-1-Ig (same clone as 67223-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL17 antibody (67223-1-Ig, Clone: 1C5E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67223-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

67223-1-PBS cible RPL17 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RPL17 Protéine recombinante Ag5393
• Nom complet: ribosomal protein L17
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 20-23 kDa
• Numéro d’acquisition GenBank: BC066323
• Symbole du gène: RPL17
• Identification du gène (NCBI): 6139
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL17 gene encodes a ribosomal protein that is a component of the 60S subunit belonging to the L22P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL23 because the encoded protein shares amino acid identity with ribosomal protein L23 from Halobacterium marismortui; however, its official symbol is RPL17.

{{ptg:RelatedPrimaryAntibodies}}