Anticorps Polyclonal de lapin anti-RPL18

RPL18 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 17029-1-AP

Synonymes

60S ribosomal protein L18, ribosomal protein L18, RPL18

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Galerie de données de validation

• 

  WB analysis of HeLa using 17029-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17029-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17029-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17029-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17029-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

17029-1-AP cible RPL18 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPL18 Protéine recombinante Ag10474
• Nom complet: ribosomal protein L18
• Masse moléculaire calculée: 188 aa, 22 kDa
• Poids moléculaire observé: 22-26 kDa
• Numéro d’acquisition GenBank: BC009708
• Symbole du gène: RPL18
• Identification du gène (NCBI): 6141
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
pig	WB	Virus Res; Up-regulated 60S ribosomal protein L18 in PEDV N protein-induced S-phase arrested host cells promotes viral replication; Authors - Qinghe Zhu ; KD Validated
human	WB	iScience; TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment; Authors - Magdalena Wolczyk
mouse	WB	Biomaterials; P-Pev: micelle-like complexes transformed from tumor extracellular vesicles by PEG-PE for personalized therapeutic tumor vaccine; Authors - Hongjian Tian

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Katie (Verified Customer) (05-23-2022)

Gives appropriate sized band in western blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T cells - mouse

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