Anticorps Polyclonal de lapin anti-RPL24

• Phare
• Validé par KD/KO

RPL24 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications(21)

Avis (1)

N° de cat : 17082-1-AP

Synonymes

ribosomal protein L24, Large ribosomal subunit protein eL24, 60S ribosomal protein L30, 60S ribosomal protein L24

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Galerie de données de validation

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  WB analysis using 17082-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17082-1-AP (RPL24 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 17082-1-AP

  IP result of anti-RPL24 (IP:17082-1-AP, 4ug; Detection:17082-1-AP 1:20000) with HEK-293 cells lysate 920 ug.

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  IHC staining of human placenta using 17082-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17082-1-AP (RPL24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 17082-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17082-1-AP (RPL24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17082-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17082-1-AP (RPL24 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17082-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17082-1-AP (RPL24 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 17082-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17082-1-AP (RPL24 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 17082-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17082-1-AP (RPL24 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 17082-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17082-1-AP (RPL24 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17082-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17082-1-AP (RPL24 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17082-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17082-1-AP (RPL24 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17082-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17082-1-AP (RPL24 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 17082-1-AP

  Immunofluorescent analysis of Hela cells, using RPL24 antibody 17082-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules Jurkat
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu placentaire humain, tissu hépatique humain, tissu ovarien humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

17082-1-AP cible RPL24 dans les applications de WB, IHC, IF/ICC, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris, xénope
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPL24 Protéine recombinante Ag7085
• Nom complet: ribosomal protein L24
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 21-23 kDa
• Numéro d’acquisition GenBank: BC000690
• Symbole du gène: RPL24
• Identification du gène (NCBI): 6152
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL24 is one component of the large (60S) subunits that promote the translation of uORF-containing mRNAsgene The mutation in Rpl24 result in impairment of mRNA splicing and L24 production, which in turn affects ribosome biogenesis, protein synthesis and the cell cycle. PMID:20799971]. Also RPL24 (ribosomal protein L24) is a key factor for translation reinitiation of downstream ORFs on the polycistronic cauliflower mosaic virus 35S RNA transcription unit, and may have a role in gynoecuim development.[PMID:15270688]

Publications

Species	Application	Title
human	WB	Cell; Ribosome ADP-ribosylation inhibits translation and maintains proteostasis in cancers.; Authors - Sridevi Challa ; KD Validated
human	WB	Cell Stem Cell; HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis.; Authors - Kaosheng Lv
	IF	Neuron; Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome.; Authors - Roberta Cagnetta
	WB	Dev Cell; Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions; Authors - Max Koppers
human	IHC, WB	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
	WB,IP	Cell Mol Life Sci; Ribosomal protein L24 mediates mammalian microRNA processing in an evolutionarily conserved manner; Authors - Yonat Tzur

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Erica (Verified Customer) (05-15-2019)

The antibody works very well for WB in both Human HEK and MEF cells. I used 1:2000 dilution in 2%BSA, and it always work well. It doesn't work very well for IP though..

• Applications: Western Blot, Immunoprecipitation,
• Cell Tissue Type: Mammalian cells

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