Anticorps Polyclonal de lapin anti-RPL27

RPL27 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 14980-1-AP

Synonymes

60S ribosomal protein L27, ribosomal protein L27, RPL27

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Galerie de données de validation

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  WB analysis of mouse liver using 14980-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14980-1-AP (RPL27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 14980-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14980-1-AP (RPL27 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 14980-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14980-1-AP (RPL27 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 14980-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14980-1-AP (RPL27 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

14980-1-AP cible RPL27 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPL27 Protéine recombinante Ag6893
• Nom complet: ribosomal protein L27
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC001700
• Symbole du gène: RPL27
• Identification du gène (NCBI): 6155
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RPL27, also named as 60S ribosomal protein L27, is a 136 amino acid protein, which contains one KOW domain and belongs to the ribosomal protein L27e family. The function of RPL27 is still non-known. RPL27 may play a role in protein translation. Like most ribosomal proteins, Ribosomal Protein L27 exists as multiple processed pseudogenes that are scattered throughout the genome.

Publications

Species	Application	Title
human	IHC	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
rat	WB,IHC	J Orthop Translat; Comparative proteomic analysis identifies differentially expressed proteins and reveals potential mechanisms of traumatic heterotopic ossification progression.; Authors - Zhenyuan Wei
rat	WB	Physiol Rep; Aldosterone and angiotensin II induce protein aggregation in renal proximal tubules.; Authors - Muhammad U Cheema
mouse	WB	EMBO Rep; miR-132 suppresses transcription of ribosomal proteins to promote protective Th1 immunity.; Authors - James P Hewitson
rat	WB	Brain Sci; Identification and Validation of Novel Potential Pathogenesis and Biomarkers to Predict the Neurological Outcome after Cardiac Arrest; Authors - Qiang Zhang
mouse	IF	Nat Struct Mol Biol; Nucleolus assembly impairment leads to two-cell transcriptional repression via NPM1-mediated PRC2 recruitment; Authors - Dingfeng Li

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