Anticorps Recombinant de lapin anti-RPL29

RPL29 Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240062F9

N° de cat : 83377-1-PBS

Synonymes

Large ribosomal subunit protein eL29, HUMRPL29, HIP, Cell surface heparin-binding protein HIP, 60S ribosomal protein L29

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Galerie de données de validation

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  WB analysis using 83377-1-RR (same clone as 83377-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83377-1-RR (RPL29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83377-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83377-1-RR (same clone as 83377-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL29 antibody (83377-1-RR, Clone: 240062F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83377-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83377-1-RR (same clone as 83377-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL29 antibody (83377-1-RR, Clone: 240062F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83377-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83377-1-RR (same clone as 83377-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPL29 Recombinant antibody (83377-1-RR, Clone:240062F9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83377-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83377-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83377-1-RR against Human RPL29 were performed. The affinity constant is 0.254 nM.

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Informations sur le produit

83377-1-PBS cible RPL29 dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RPL29 Protéine recombinante Ag34100
• Nom complet: ribosomal protein L29
• Masse moléculaire calculée: 159 aa, 18 kDa
• Numéro d’acquisition GenBank: BC008926
• Symbole du gène: RPL29
• Identification du gène (NCBI): 6159
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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