Anticorps Polyclonal de lapin anti-RPL3

• Phare
• Validé par KD/KO

RPL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11005-1-PBS

Synonymes

60S ribosomal protein L3, HIV-1 TAR RNA-binding protein B, Large ribosomal subunit protein uL3, OK/SW-cl.32, ribosomal protein L3

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Galerie de données de validation

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  WB analysis of HeLa using 11005-1-AP (same clone as 11005-1-PBS)

  WB result of RPL3 antibody (11005-1-AP, 1:2000) with si-control and si-RPL3 transfected HeLa cells.. This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

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  WB analysis using 11005-1-AP (same clone as 11005-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11005-1-AP (RPL3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 11005-1-AP (same clone as 11005-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11005-1-AP (RPL3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 11005-1-AP (same clone as 11005-1-PBS)

  IP result of anti-RPL3 (IP:11005-1-AP, 4ug; Detection:11005-1-AP 1:10000) with HepG2 cells lysate 1480 ug. This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11005-1-AP (same clone as 11005-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11005-1-AP (RPL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11005-1-AP (same clone as 11005-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11005-1-AP (RPL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11005-1-AP (same clone as 11005-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPL3 antibody (11005-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11005-1-PBS cible RPL3 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPL3 Protéine recombinante Ag1436
• Nom complet: ribosomal protein L3
• Masse moléculaire calculée: 46/27 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC012786
• Symbole du gène: RPL3
• Identification du gène (NCBI): 6122
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. This is a rabbit polyclonal antibody raised against the C terminus of human RPL3.

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