Anticorps Polyclonal de lapin anti-RPL35

RPL35 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 14826-1-AP

Synonymes

60S ribosomal protein L35, ribosomal protein L35, RPL35

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Galerie de données de validation

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  WB analysis of mouse heart using 14826-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of mouse heart using 14826-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse skeletal muscle using 14826-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 14826-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 14826-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse skeletal muscle using 14826-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  IHC staining of human liver cancer using 14826-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14826-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 14826-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 14826-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 14826-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 14826-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 14826-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 14826-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14826-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14826-1-AP (RPL35 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HeLa, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu pancréatique de rat, tissu de cancer de la thyroïde humain, tissu de cancer de l'estomac humain, tissu de cancer du foie humain, tissu de cancer du pancréas humain, tissu pancréatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

14826-1-AP cible RPL35 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPL35 Protéine recombinante Ag6615
• Nom complet: ribosomal protein L35
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC000348
• Symbole du gène: RPL35
• Identification du gène (NCBI): 11224
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	J Cell Sci; Drp1 regulates transcription of ribosomal protein genes in embryonic hearts.; Authors - Qiancong Zhao