Anticorps Monoclonal anti-Ribosomal protein L4

Ribosomal protein L4 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(5)

CloneNo.

3C7A8

N° de cat : 67028-1-Ig

Synonymes

RPL4, 3C7A8, 60S ribosomal protein L1, 60S ribosomal protein L4, RPL 4

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Galerie de données de validation

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  WB analysis using 67028-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67028-1-Ig (Ribosomal protein L4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67028-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67028-1-Ig (Ribosomal protein L4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 67028-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67028-1-Ig (Ribosomal protein L4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 67028-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67028-1-Ig (Ribosomal protein L4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67028-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ribosomal protein L4 antibody (67028-1-Ig, Clone: 3C7A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 67028-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ribosomal protein L4 antibody (67028-1-Ig, Clone: 3C7A8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

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  FC experiment of HepG2 using 67028-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Ribosomal protein L4 (67028-1-Ig, Clone:3C7A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, cellules RAW 264.7
• Résultats positifs en IHC: tissu de lymphome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

67028-1-Ig cible Ribosomal protein L4 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Ribosomal protein L4 Protéine recombinante Ag28728
• Nom complet: ribosomal protein L4
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC009888
• Symbole du gène: Ribosomal protein L4
• Identification du gène (NCBI): 6124
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.

Publications

Species	Application	Title
human	WB,IP	Nat Commun; The short isoform of the host antiviral protein ZAP acts as an inhibitor of SARS-CoV-2 programmed ribosomal frameshifting.; Authors - Matthias M Zimmer
mouse	WB	Dev Cell; DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice; Authors - Dingfeng Zou
mouse	WB	Cell Rep; FMRP Control of Ribosome Translocation Promotes Chromatin Modifications and Alternative Splicing of Neuronal Genes Linked to Autism.; Authors - Sneha Shah
human	WB,IP,CoIP	Exp Cell Res; PRC1 promotes ovarian cancer progression by binding to RPL4 and increasing MDM2-mediated p53 ubiquitination; Authors - Yinyin Xu
rat	WB	Free Radic Biol Med; Mitochondrial Electron Transfer Chain Activation in Atrophic Muscle: Implications for Calcium Signaling and Chondrocyte Apoptosis in Osteoarthritis; Authors - Ailin Li

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