Anticorps Polyclonal de lapin anti-RPL7A

RPL7A Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 15340-1-AP

Synonymes

60S ribosomal protein L7a, PLA X polypeptide, ribosomal protein L7a, RPL7A, SURF 3, SURF3, Surfeit locus protein 3, TRUP

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Galerie de données de validation

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  WB analysis of MCF-7 using 15340-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15340-1-AP (RPL7A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15340-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15340-1-AP (RPL7A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 15340-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15340-1-AP (RPL7A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15340-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15340-1-AP (RPL7A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15340-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15340-1-AP (RPL7A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 15340-1-AP

  IP Result of anti-RPL7A (IP:15340-1-AP, 4ug; Detection:15340-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human breast cancer using 15340-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15340-1-AP (RPL7A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15340-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15340-1-AP (RPL7A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15340-1-AP

  Immunofluorescent analysis of HepG2 cells, using RPL7A antibody 15340-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules MCF7, cellules MCF-7, tissu hépatique de souris, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

15340-1-AP cible RPL7A dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPL7A Protéine recombinante Ag7543
• Nom complet: ribosomal protein L7a
• Masse moléculaire calculée: 266 aa, 30 kDa
• Poids moléculaire observé: 30-32 kDa
• Numéro d’acquisition GenBank: BC005128
• Symbole du gène: RPL7A
• Identification du gène (NCBI): 6130
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribosomal proteins are a major component of ribosomes, which catalyze protein synthesis. RPL7a, which is a component of the 60S large ribosomal subunit, has additional functions involved in cell growth and differentiation that occur via interaction with human thyroid hormone receptor (THR) and retinoic acid receptor (RAR) and in turn inhibit the activities of the two nuclear hormone receptors [PMID:21505254].

Publications

Species	Application	Title
mouse	WB, IF	Cell; Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms.; Authors - Takashi Mino
human	IP	Mol Cell; Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover.; Authors - Dianrong Li
human	WB	Acta Neuropathol Commun; Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease; Authors - Suman Rimal
human	WB	Nucleic Acids Res; The induction of p53 correlates with defects in the production, but not the levels, of the small ribosomal subunit and stalled large ribosomal subunit biogenesis; Authors - Matthew John Eastham
human	WB	Am J Cancer Res; UBE2S targets RPL26 for ubiquitination and degradation to promote non-small cell lung cancer progression via regulating c-Myc; Authors - Dalian Gong
mouse	WB	J Exp Med; Phosphorylation-dependent Regnase-1 release from endoplasmic reticulum is critical in IL-17 response.; Authors - Hiroki Tanaka