Anticorps Polyclonal de lapin anti-Ribophorin I

• Phare
• Validé par KD/KO

Ribophorin I Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(20)

N° de cat : 12894-1-AP

Synonymes

RPN1, RPN-I, Ribophorin-1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit

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Galerie de données de validation

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  WB analysis using 12894-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12894-1-AP (RPN1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12894-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12894-1-AP (RPN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 12894-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12894-1-AP (RPN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 12894-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12894-1-AP (RPN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 12894-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12894-1-AP (RPN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 12894-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12894-1-AP (RPN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules HeLa, cellules K-562
• Résultats positifs en IHC: tissu pulmonaire de souris, tissu hépatique de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IHC: See 3 publications below

Informations sur le produit

12894-1-AP cible Ribophorin I dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, levure, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Ribophorin I Protéine recombinante Ag3576
• Nom complet: ribophorin I
• Masse moléculaire calculée: 69 kDa
• Poids moléculaire observé: 66-69 kDa
• Numéro d’acquisition GenBank: BC010839
• Symbole du gène: RPN1
• Identification du gène (NCBI): 6184
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribophorin I, also named as RPN1, is a key protein component of the oligosaccharyltransferase (OST) complex, which is embedded in the membrane of the rough endoplasmic reticulum (RER) (PMID: 38373565). It plays a crucial role in the process of N-linked glycosylation, a post-translational modification essential for the proper folding, stability, and function of many secretory and membrane-bound proteins (PMID: 17264154). Ribophorin I, along with Ribophorin II, forms a heterodimeric complex that facilitates the transfer of oligosaccharides from a lipid-linked donor to specific asparagine residues on nascent polypeptide chains (PMID: 30940778).

Publications

Species	Application	Title
yeast	WB	Nature; Reconfiguration of the proteasome during chaperone-mediated assembly.; Authors - Soyeon Park
mouse	WB	Cell Metab; Protein O-GlcNAcylation and hexokinase mitochondrial dissociation drive heart failure with preserved ejection fraction; Authors - Yuki Tatekoshi
mouse	WB	Nat Cell Biol; Manganese regulation of COPII condensation controls circulating lipid homeostasis; Authors - Xiao Wang
human	WB	Sci Adv; The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress.; Authors - Chatchai Phoomak
human	WB	Nat Chem Biol; Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells.; Authors - Cecilia Lopez-Sambrooks
rat	WB	Int J Mol Sci; Impaired 26S Proteasome Assembly Precedes Neuronal Loss in Mutant UBQLN2 Rats.; Authors - Wenjuan Zhang

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