Anticorps Recombinant de lapin anti-Ribophorin I

• Phare
• Validé par KD/KO

Ribophorin I Recombinant Antibody for WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240044D4

N° de cat : 83250-2-PBS

Synonymes

RPN1, 240044D4, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Ribophorin-1

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Galerie de données de validation

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  WB analysis using 83250-2-RR (same clone as 83250-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83250-2-RR (RPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83250-2-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 83250-2-RR (same clone as 83250-2-PBS)

  WB result of Ribophorin I antibody (83250-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ribophorin I transfected HepG2 cells. This data was developed using the same antibody clone with 83250-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00231-3

  Sandwich ELISA standard curve of MP00231-3, Human Ribophorin I Recombinant Matched Antibody Pair - PBS only. 83250-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3576. 83250-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP00231-3

  The mean Ribophorin I concentration was determined to be 145.4 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load and 11.0 ng/mL in A431 cell extract based on a 1.5 mg/mL extract load.

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  IHC staining of human stomach cancer using 83250-2-RR (same clone as 83250-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83250-2-RR (Ribophorin I antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83250-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83250-2-RR (same clone as 83250-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPN1 Recombinant antibody (83250-2-RR, Clone:240044D4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83250-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83250-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83250-2-PBS against Human Ribophorin I was performed. The affinity constant is 1.69 nM.

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  Affinity and Kinetic Characterization of 83250-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83250-2-RR against Human RPN1 were performed. The affinity constant is 0.154 uM.

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Informations sur le produit

83250-2-PBS cible Ribophorin I dans les applications de WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Ribophorin I Protéine recombinante Ag3576
• Nom complet: ribophorin I
• Masse moléculaire calculée: 69 kDa
• Poids moléculaire observé: 66-69 kDa
• Numéro d’acquisition GenBank: BC010839
• Symbole du gène: RPN1
• Identification du gène (NCBI): 6184
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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