Anticorps Polyclonal de lapin anti-RPS12

• Phare
• Validé par KD/KO

RPS12 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(17)

N° de cat : 16490-1-AP

Synonymes

40S ribosomal protein S12, ribosomal protein S12, Small ribosomal subunit protein eS12

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 16490-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16490-1-AP (RPS12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16490-1-AP

  WB result of RPS12 antibody (16490-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPS12 transfected HepG2 cells.

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  WB analysis using 16490-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16490-1-AP (RPS12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16490-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16490-1-AP (RPS12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16490-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16490-1-AP (RPS12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16490-1-AP

  IP result of anti-RPS12 (IP:16490-1-AP, 4ug; Detection:16490-1-AP 1:1000) with HeLa cells lysate 2920 ug.

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  IHC staining of human colon cancer using 16490-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16490-1-AP (RPS12 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 16490-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16490-1-AP (RPS12 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 16490-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using RPS12 antibody (16490-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HeLa, cellules HepG2, cellules MCF-7
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

16490-1-AP cible RPS12 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Drosophile, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPS12 Protéine recombinante Ag9615
• Nom complet: ribosomal protein S12
• Masse moléculaire calculée: 132 aa, 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC017321
• Symbole du gène: RPS12
• Identification du gène (NCBI): 6206
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RPS12 (ribosomal protein S12) is a plastid ribosomal protein which is a part of the 30S ribosomal subunit. Together with S4 and S5 plays an important role in translational accuracy.This antibody specifically recognizes the 15kd RPS12 protein.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Functional screen identifies RBM42 as a mediator of oncogenic mRNA translation specificity; Authors - Joanna R Kovalski
mouse	WB	Mol Cell; RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages; Authors - Teodorus Theo Susanto
	WB	Dev Cell; Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions; Authors - Max Koppers
		Dev Cell; Progressive Differentiation and Instructive Capacities of Amniotic Fluid and Cerebrospinal Fluid Proteomes following Neural Tube Closure.; Authors - Kevin F Chau
mouse	IHC	Elife; Downregulation of ribosome biogenesis during early forebrain development.; Authors - Kevin F Chau
human	WB	Sci Signal; Repurposing colforsin daropate to treat MYC-driven high-grade serous ovarian carcinomas; Authors - Matthew J Knarr

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