Anticorps Monoclonal anti-RPS12

• Phare
• Validé par KD/KO

RPS12 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A3D1

N° de cat : 67683-1-PBS

Synonymes

1A3D1, 40S ribosomal protein S12, ribosomal protein S12, Small ribosomal subunit protein eS12

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Galerie de données de validation

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  WB analysis using 67683-1-Ig (same clone as 67683-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67683-1-Ig (RPS12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67683-1-Ig (same clone as 67683-1-PBS)

  WB result of RPS12 antibody (67683-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPS12 transfected HepG2 cells. This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

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  WB analysis of NCI-H1299 using 67683-1-Ig (same clone as 67683-1-PBS)

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67683-1-Ig (RPS12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67683-1-Ig (same clone as 67683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67683-1-Ig (same clone as 67683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67683-1-Ig (same clone as 67683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67683-1-Ig (same clone as 67683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67683-1-Ig (same clone as 67683-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS12 antibody (67683-1-Ig, Clone: 1A3D1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67683-1-PBS cible RPS12 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RPS12 Protéine recombinante Ag9649
• Nom complet: ribosomal protein S12
• Masse moléculaire calculée: 132 aa, 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC017321
• Symbole du gène: RPS12
• Identification du gène (NCBI): 6206
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RPS12 (ribosomal protein S12) is a plastid ribosomal protein which is a part of the 30S ribosomal subunit. Together with S4 and S5 plays an important role in translational accuracy.

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