Anticorps Polyclonal de lapin anti-RPS20

• Phare
• Validé par KD/KO

RPS20 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 15692-1-AP

Synonymes

40S ribosomal protein S20, ribosomal protein S20, RPS20

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Galerie de données de validation

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  WB analysis of MCF-7 using 15692-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15692-1-AP (RPS20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15692-1-AP

  WB result of RPS20 antibody (15692-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPS20 transfected HepG2 cells.

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  WB analysis of HepG2 using 15692-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15692-1-AP (RPS20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15692-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15692-1-AP (RPS20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15692-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15692-1-AP (RPS20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules MCF7, cellules HEK-293, cellules HepG2, cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Informations sur le produit

15692-1-AP cible RPS20 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPS20 Protéine recombinante Ag8257
• Nom complet: ribosomal protein S20
• Masse moléculaire calculée: 13 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC007507
• Symbole du gène: RPS20
• Identification du gène (NCBI): 6224
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS20 is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Functional screen identifies RBM42 as a mediator of oncogenic mRNA translation specificity; Authors - Joanna R Kovalski
mouse	WB	Mol Cell; RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages; Authors - Teodorus Theo Susanto
human	WB	bioRxiv; Functional screen for mediators of onco-mRNA translation specificity; Authors - Joanna R Kovalski
human,mouse	WB,IHC	Oncol Rep; Biochemical and clinical effects of RPS20 expression in renal clear cell carcinoma; Authors - Cheng Shen ; KD Validated
mouse	WB	J Pineal Res; Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin; Authors - Jingming Ren
human,mouse	WB	bioRxiv; RAPIDASH: A tag-free enrichment of ribosome-associated proteins reveals compositional dynamics in embryonic tissues and stimulated macrophages; Authors - Teodorus Theo Susanto

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