Anticorps Polyclonal de lapin anti-RPS21

• Phare
• Validé par KD/KO

RPS21 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 16946-1-AP

Synonymes

Small ribosomal subunit protein eS21, ribosomal protein S21, 40S ribosomal protein S21

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Galerie de données de validation

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  WB analysis using 16946-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 16946-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16946-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 16946-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16946-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16946-1-AP (RPS21 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16946-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16946-1-AP (RPS21 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16946-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPS21 antibody (16946-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules SGC-7901, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below

Informations sur le produit

16946-1-AP cible RPS21 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPS21 Protéine recombinante Ag10557
• Nom complet: ribosomal protein S21
• Masse moléculaire calculée: 81 aa, 9 kDa
• Poids moléculaire observé: 9 kDa
• Numéro d’acquisition GenBank: BC018140
• Symbole du gène: RPS21
• Identification du gène (NCBI): 6227
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit.

Publications

Species	Application	Title
mouse	WB	Endocrinology; Wnt16 Promotes Vascular Smooth Muscle Contractile Phenotype and Function via Taz (Wwtr1) Activation in Male LDLR-/- Mice; Authors - Abraham Behrmann
		J Cell Sci; Drp1 regulates transcription of ribosomal protein genes in embryonic hearts.; Authors - Qiancong Zhao
human	IHC	Oncol Lett; Identification of the key genes and pathways in prostate cancer.; Authors - Shutong Fan
human	WB,IHC	Med Oncol; Identification of candidate diagnostic and prognostic biomarkers for human prostate cancer: RPL22L1 and RPS21.; Authors - Zumu Liang ; KD Validated
mouse	IHC	Reprod Sci; Upregulated Ribosomal Pathway Impairs Follicle Development in a Polycystic Ovary Syndrome Mouse Model: Differential Gene Expression Analysis of Oocytes; Authors - Natsuki Nakanishi

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