Anticorps Polyclonal de lapin anti-RPS25

RPS25 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 23599-1-AP

Synonymes

40S ribosomal protein S25, ribosomal protein S25, RPS25

---

Galerie de données de validation

• 

  WB analysis using 23599-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23599-1-AP (RPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 23599-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23599-1-AP (RPS25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 23599-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 23599-1-AP (RPS25 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 23599-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23599-1-AP (RPS25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 23599-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 23599-1-AP (RPS25 Antibody) at dilution of 1:50.

• 

  IF Staining of MCF-7 using 23599-1-AP

  Immunofluorescent analysis of MCF-7 cells using 23599-1-AP (RPS25 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules K-562, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

23599-1-AP cible RPS25 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPS25 Protéine recombinante Ag7890
• Nom complet: ribosomal protein S25
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 15-17 kDa
• Numéro d’acquisition GenBank: BC004986
• Symbole du gène: RPS25
• Identification du gène (NCBI): 6230
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribosomal protein S25 (RPS25) is a novel MDM2 interacting protein. It may be involved in viral replication of Dicistroviridae and hepatitis C viruses. RPS25 is overexpressed in human leukemia cells exhibiting adriamycin resistance. It may have a role in p53-mediated apoptosis and cell-cycle arrest. The expected MW of RPS25 is about 15-17kDa. Catalog# 23599-1-AP is a rabbit polyclonal antibody raised against full-length RPS25, and is recommended for ELISA, Western blotting.

Publications

Species	Application	Title
human	WB	ACS Nano; Platelet-Derived Apoptotic Vesicles Promote Bone Regeneration via Golgi Phosphoprotein 2 (GOLPH2)-AKT Signaling Axis; Authors - Yuhe Jiang
human	WB	Mol Cell Proteomics; Identification and characterization of potential biomarkers by quantitative tissue proteomics of primary lung adenocarcinoma.; Authors - Chiung-Hung Hsu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

H (Verified Customer) (07-09-2020)

This antibody works well for screening of crispr knock-in. There were a couple non-specific bands above though.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HCT116

RPS25 Antibody Western Blot validation (1:2000 dilution) in HCT116 (Cat no:23599-1-AP)

{{ptg:RelatedPrimaryAntibodies}}