Anticorps Polyclonal de lapin anti-RPS3

• Phare
• Validé par KD/KO

RPS3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(23)

Avis (2)

N° de cat : 11990-1-AP

Synonymes

ribosomal protein S3, OK/SW-cl.26, FLJ27450, FLJ26283, EC:4.2.99.18

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Galerie de données de validation

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  WB analysis using 11990-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11990-1-AP (RPS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 11990-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 11990-1-AP (RPS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11990-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11990-1-AP (RPS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 11990-1-AP

  IP result of anti-RPS3 (IP:11990-1-AP, 3ug; Detection:11990-1-AP 1:600) with mouse testis tissue lysate 4000ug.

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  IHC staining of rat liver using 11990-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11990-1-AP (RPS3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 11990-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11990-1-AP (RPS3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 11990-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RPS3 antibody (11990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules MCF-7, cellules NIH/3T3, cellules PC-12, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu hépatique de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 18 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Informations sur le produit

11990-1-AP cible RPS3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPS3 Protéine recombinante Ag3918
• Nom complet: ribosomal protein S3
• Masse moléculaire calculée: 26 aa, 7 kDa
• Poids moléculaire observé: 26.7 kDa
• Numéro d’acquisition GenBank: BC034149
• Symbole du gène: RPS3
• Identification du gène (NCBI): 6188
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival.

Publications

Species	Application	Title
human	WB	Nat Commun; Kaposi's sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs; Authors - James C Murphy
human	WB	Mol Cell; A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation; Authors - Raja H Ali
mouse	IF	Nat Commun; Cilia locally synthesize proteins to sustain their ultrastructure and functions.; Authors - Kai Hao
human	WB	Curr Biol; RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.; Authors - Long Jiang
pig	IF	J Anim Sci Biotechnol; Glyphosate exposure deteriorates oocyte meiotic maturation via induction of organelle dysfunctions in pigs.; Authors - Chunhua Xing
human	WB,IHC	Front Oncol; RPS3 Promotes the Metastasis and Cisplatin Resistance of Adenoid Cystic Carcinoma.; Authors - Xi Rui

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (12-16-2021)

This antibody recognized a clean band at 27 kd.

• Applications: Western Blot

FH

Huanzhou (Verified Customer) (11-26-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Cl-16

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