Anticorps Recombinant de lapin anti-RPS3

RPS3 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242504F3

N° de cat : 84947-4-PBS

Synonymes

242504F3, 40S ribosomal protein S3, EC:4.2.99.18, FLJ26283, FLJ27450

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Galerie de données de validation

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  WB analysis using 84947-4-RR (same clone as 84947-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84947-4-RR (RPS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84947-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84947-4-RR (same clone as 84947-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84947-4-RR (RPS3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84947-4-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 84947-4-RR (same clone as 84947-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RPS3 antibody (84947-4-RR, Clone: 242504F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84947-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84947-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84947-4-RR against Human RPS3 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84947-4-PBS cible RPS3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RPS3 Protéine recombinante Ag7346
• Nom complet: ribosomal protein S3
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC003137
• Symbole du gène: RPS3
• Identification du gène (NCBI): 6188
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival.

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