Anticorps Polyclonal de lapin anti-RSK3

• Phare
• Validé par KD/KO

RSK3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 14446-1-AP

Synonymes

HU 2, MAPKAP kinase 1c, MAPKAPK 1c, MAPKAPK1C, p90 RSK 2, p90 RSK3, p90RSK2, pp90RSK3, Ribosomal S6 kinase 3, RPS6KA2, RSK, RSK 3, RSK3, S6K alpha, S6K alpha 2, S6K alpha2

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 14446-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14446-1-AP (RSK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14446-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14446-1-AP (RSK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14446-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14446-1-AP (RSK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 14446-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14446-1-AP (RSK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14446-1-AP

  IP Result of anti-RSK3 (IP:14446-1-AP, 4ug; Detection:14446-1-AP 1:800) with HeLa cells lysate 4000ug.

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  IHC staining of human lung cancer using 14446-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14446-1-AP (RSK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14446-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14446-1-AP (RSK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14446-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14446-1-AP (RSK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14446-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14446-1-AP (RSK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14446-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14446-1-AP (RSK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14446-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14446-1-AP (RSK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HeLa, cellules MCF-7, cellules MDA-MB-231
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

14446-1-AP cible RSK3 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RSK3 Protéine recombinante Ag5862
• Nom complet: ribosomal protein S6 kinase, 90kDa, polypeptide 2
• Masse moléculaire calculée: 83 kDa
• Poids moléculaire observé: 83 kDa
• Numéro d’acquisition GenBank: BC002363
• Symbole du gène: RPS6KA2
• Identification du gène (NCBI): 6196
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RSK3, also named as RPS6KA2, belongs to the protein kinase superfamily. RSK3 is a serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. In addition RSK3 may function as tumor suppressor in epithelial ovarian cancer cells(Uniprot). The molecular weight of RSK3 is 83 kDa.

Publications

Species	Application	Title
human	WB	Theranostics; Kartogenin hydrolysis product 4-aminobiphenyl distributes to cartilage and mediates cartilage regeneration.; Authors - Shuai Zhang
mouse	WB,IHC	Theranostics; RSK-3 promotes cartilage regeneration via interacting with rpS6 in cartilage stem/progenitor cells.; Authors - Shuai Zhang ; KD Validated
human	IHC	Epigenomics; Multiomics analysis identifies key genes and pathways related to N6-methyladenosine RNA modification in ovarian cancer.; Authors - Haoya Xu
human	CoIP	Cell Death Discov; MYSM1 induces apoptosis and sensitizes TNBC cells to cisplatin via RSK3-phospho-BAD pathway.; Authors - Xiaolin Guan
human	WB	J Med Chem; Discovery, Optimization, and Structure-Activity Relationship Study of Novel and Potent RSK4 Inhibitors as Promising Agents for the Treatment of Esophageal Squamous Cell Carcinoma; Authors - Yuan Yuan