Anticorps Polyclonal de lapin anti-RPS9

• Phare
• Validé par KD/KO

RPS9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(10)

Avis (1)

N° de cat : 18215-1-AP

Synonymes

40S ribosomal protein S9, ribosomal protein S9, Small ribosomal subunit protein uS4

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Galerie de données de validation

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  WB analysis of mouse uterus using 18215-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 18215-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 18215-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18215-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 18215-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RPS9 antibody (18215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu utérin de souris, cellules HeLa, tissu ovarien de souris
• Résultats positifs en IHC: tissu pulmonaire humain, tissu cutané humain, tissu d'estomac de souris, tissu ovarien humain, tissu pancréatique de souris, tissu pulmonaire de souris, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

18215-1-AP cible RPS9 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPS9 Protéine recombinante Ag12945
• Nom complet: ribosomal protein S9
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC000802
• Symbole du gène: RPS9
• Identification du gène (NCBI): 6203
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Dev Cell; Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.; Authors - Yuqing Lei
human	WB	Proc Natl Acad Sci U S A; Preventing translational inhibition from ribosomal protein insufficiency by a herpes simplex virus-encoded ribosome-associated protein.; Authors - Elizabeth I Vink ; KD Validated
human	IHC	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
human	WB	Sci Rep; Down-regulation of HSP60 Suppresses the Proliferation of Glioblastoma Cells via the ROS/AMPK/mTOR Pathway.; Authors - Haiping Tang
human	WB	Front Immunol; Gradient Boosting Machine Learning Model for Defective Endometrial Receptivity Prediction by Macrophage-Endometrium Interaction Modules.; Authors - Bohan Li
human	WB	Genes (Basel); Transcriptional Regulation of CD40 Expression by 4 Ribosomal Proteins via a Functional SNP on a Disease-Associated CD40 Locus.; Authors - Meijuan Zou ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wei (Verified Customer) (01-30-2020)

Single, strong band as predicted size 25kDa

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

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