Anticorps Polyclonal de lapin anti-Raptor

• Phare
• Validé par KD/KO

Raptor Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF/ICC, IP, ELISA, PLA

Conjugaison

Non conjugué

Publications(52)

N° de cat : 20984-1-AP

Synonymes

KIAA1303, TOR, RPTOR, Regulatory-associated protein of mTOR, p150 target of rapamycin

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Galerie de données de validation

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  WB analysis of HepG2 cells using 20984-1-AP

  WB result of Raptor antibody (20984-1-AP, 1:500) with si-control and si-Raptor transfected HepG2 cells.

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  WB analysis using 20984-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20984-1-AP (Raptor antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 20984-1-AP

  IP result of anti-Raptor (IP:20984-1-AP, 4ug; Detection:20984-1-AP 1:4000) with HEK-293 cells lysate 1400 ug.

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  IF Staining of U2OS using 20984-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Raptor antibody (20984-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, tissu hépatique de souris
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 42 publications below
• IHC: See 2 publications below
• IF: See 11 publications below
• IP: See 3 publications below

Informations sur le produit

20984-1-AP cible Raptor dans les applications de WB, IHC, IF/ICC, IP, ELISA, PLA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, Lapin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: raptor
• Masse moléculaire calculée: 149 kDa
• Poids moléculaire observé: 130-150 kDa
• Numéro d’acquisition GenBank: NM_020761
• Symbole du gène: Raptor
• Identification du gène (NCBI): 57521
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RPTOR, also named as KIAA1303 and RAPTOR Belongs to the WD repeat RAPTOR family. It is involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. The antibody is specific to RPTOR.

Publications

Species	Application	Title
mouse	WB	Nat Neurosci; A glycolytic shift in Schwann cells supports injured axons.; Authors - Elisabetta Babetto
human	WB	Mol Cell; mTORC1 activity licenses its own release from the lysosomal surface; Authors - Aishwarya Acharya
mouse	WB,IP	Mol Cell; Acetyl-CoA Derived from Hepatic Peroxisomal β-Oxidation Inhibits Autophagy and Promotes Steatosis via mTORC1 Activation.; Authors - Anyuan He
mouse	WB	Autophagy; Buddleoside alleviates nonalcoholic steatohepatitis by targeting the AMPK-TFEB signaling pathway; Authors - Meng Chen
mouse	IHC	Nat Commun; N7-methylguanosine tRNA modification promotes esophageal squamous cell carcinoma tumorigenesis via the RPTOR/ULK1/autophagy axis.; Authors - Hui Han
mouse	WB,IF,PLA	Nat Commun; Location-specific inhibition of Akt reveals regulation of mTORC1 activity in the nucleus.; Authors - Xin Zhou

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