Anticorps Polyclonal de lapin anti-RRAGB

• Phare
• Validé par KD/KO

RRAGB Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 13023-1-AP

Synonymes

EC:3.6.5.-, Rag B, RagB, Ras related GTP binding B, Ras related GTP binding protein B

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Galerie de données de validation

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  WB analysis using 13023-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13023-1-AP (RRAGB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 13023-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13023-1-AP (RRAGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13023-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13023-1-AP (RRAGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13023-1-AP

  IP result of anti-RRAGB (IP:13023-1-AP, 4ug; Detection:13023-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, tissu cérébral humain, tissu cérébral humain fœtal, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IP: tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

13023-1-AP cible RRAGB dans les applications de WB, IHC, IF, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RRAGB Protéine recombinante Ag4116
• Nom complet: Ras-related GTP binding B
• Masse moléculaire calculée: 346 aa, 40 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC034726
• Symbole du gène: RRAGB
• Identification du gène (NCBI): 10325
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,RIP,IHC	Mol Cancer; circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop.; Authors - Xiaomin Li
mouse	WB,IF	J Clin Invest; YAP plays a crucial role in the development of cardiomyopathy in lysosomal storage diseases.; Authors - Shohei Ikeda ; KO Validated
mouse	WB	PLoS One; Ursolic Acid Inhibits Leucine-Stimulated mTORC1 Signaling by Suppressing mTOR Localization to Lysosome.; Authors - Xiang Ou
rat	WB	Physiol Rep; Tissue-specific expression differences in Ras-related GTP-binding proteins in male rats; Authors - Gregory N Kincheloe
mouse	WB	Cell Signal; CircMRP4 orchestrates podocytes injury via the miR-499-5p/RRAGB/mTORC1 axis in diabetic kidney disease; Authors - Shujun Deng
human	WB,IP	Cell Metab; Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism; Authors - Ning Wang

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