Anticorps Polyclonal de lapin anti-RRAGD

RRAGD Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15315-1-PBS

Synonymes

Ras-related GTP-binding protein D, Ras related GTP binding D, RagD, Rag D, EC:3.6.5.-

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Galerie de données de validation

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  WB analysis using 15315-1-AP (same clone as 15315-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15315-1-AP (RRAGD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15315-1-PBS in a different storage buffer formulation.

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  IP experiment of NIH/3T3 using 15315-1-AP (same clone as 15315-1-PBS)

  IP result of anti-RRAGD (IP:15315-1-AP, 4ug; Detection:15315-1-AP 1:500) with NIH/3T3 cells lysate 1120 ug. This data was developed using the same antibody clone with 15315-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 15315-1-AP (same clone as 15315-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RRAGD antibody (15315-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 15315-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15315-1-PBS cible RRAGD dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RRAGD Protéine recombinante Ag7557
• Nom complet: Ras-related GTP binding D
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46-50 kDa
• Numéro d’acquisition GenBank: BC003088
• Symbole du gène: RRAGD
• Identification du gène (NCBI): 58528
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ras-related GTP-binding protein D (RRAGD) is a monomeric GTP or GDP binding protein, which plays a critical role in mediating amino acid stimulated mTOR signaling pathway, a key pathway in determination of cell growth and proliferation rate. Recently, RRAGD has been reported involving the progression of ovarian cancer and colon cancer.

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