Anticorps Polyclonal de lapin anti-RRAS2

RRAS2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12530-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 12530-1-AP (same clone as 12530-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12530-1-AP (RRAS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse pancreas using 12530-1-AP (same clone as 12530-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12530-1-AP (RRAS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.

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  IP experiment of NIH/3T3 using 12530-1-AP (same clone as 12530-1-PBS)

  IP result of anti-RRAS2 (IP:12530-1-AP, 4ug; Detection:12530-1-AP 1:800) with NIH/3T3 cells lysate 4000ug. This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 12530-1-AP (same clone as 12530-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12530-1-AP (RRAS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 12530-1-AP (same clone as 12530-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12530-1-AP (RRAS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12530-1-AP (same clone as 12530-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12530-1-AP (RRAS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 12530-1-AP (same clone as 12530-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12530-1-AP (RRAS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 12530-1-AP (same clone as 12530-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12530-1-AP (RRAS2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 12530-1-AP (same clone as 12530-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12530-1-AP (RRAS2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 12530-1-AP (same clone as 12530-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12530-1-AP (RRAS2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 12530-1-AP (same clone as 12530-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RRAS2 antibody (12530-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 12530-1-AP (same clone as 12530-1-PBS)

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human RRAS2 (12530-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12530-1-PBS cible RRAS2 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RRAS2 Protéine recombinante Ag3226
• Nom complet: related RAS viral (r-ras) oncogene homolog 2
• Masse moléculaire calculée: 204 aa, 23 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC013106
• Symbole du gène: RRAS2
• Identification du gène (NCBI): 22800
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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