Anticorps Recombinant de lapin anti-RREB1-Specific

RREB1-Specific Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240800B7

N° de cat : 84048-1-RR

Synonymes

RREB1, Ras-responsive element-binding protein 1, Raf-responsive zinc finger protein LZ321, LZ321, HNT

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Galerie de données de validation

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  WB analysis using 84048-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84048-1-RR (RREB1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84048-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RREB1-Specific antibody (84048-1-RR, Clone: 240800B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of U-2 OS using 84048-1-RR

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug RREB1-Specific Recombinant antibody (84048-1-RR, Clone:240800B7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HEK-293, cellules Jurkat
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): U-2 OS,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84048-1-RR cible RREB1-Specific dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ras responsive element binding protein 1
• Masse moléculaire calculée: 181 kDa
• Poids moléculaire observé: 260 kDa
• Numéro d’acquisition GenBank: NM_001003698
• Symbole du gène: RREB1
• Identification du gène (NCBI): 6239
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RREB1, also named as FINB and Zep-1, belongs to the krueppel C2H2-type zinc-finger protein family. RREB1 is a transcription factor that binds specifically to the RAS-responsive elements (RRE) of gene promoters. It may be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression. RREB1 represses the angiotensinogen gene. RREB1 can induce expression of p53 and represse p16 which associated with the expression of tumour. It has been reported that the RREB1 can be a candidate gene for type 2 diabetes associated end-stage kidney disease. To some extent, the decrease of zinc in human pancreatic adenocarcinoma is associated with the downregulation of RREB1. RREB1 has some isoforms with 250 kDa, 180-200 kDa and 140-160 kDa protein.(PMID: 21703425, 15067362, 25050557, 25027322)

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