Anticorps Monoclonal anti-RRM2

RRM2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

2A9A7

N° de cat : 67006-1-PBS

Synonymes

2A9A7, EC:1.17.4.1, R2, RR2, RR2M

---

Galerie de données de validation

• 

  WB analysis using 67006-1-Ig (same clone as 67006-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67006-1-Ig (RRM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67006-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67006-1-Ig (same clone as 67006-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67006-1-Ig (RRM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67006-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 67006-1-Ig (same clone as 67006-1-PBS)

  IP result of anti-RRM2 (IP:67006-1-Ig, 4ug; Detection:67006-1-Ig 1:2000) with HEK-293 cells lysate 1470 ug. This data was developed using the same antibody clone with 67006-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 67006-1-Ig (same clone as 67006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67006-1-Ig (RRM2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67006-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67006-1-Ig (same clone as 67006-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RRM2 antibody (67006-1-Ig, Clone: 2A9A7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67006-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

67006-1-PBS cible RRM2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RRM2 Protéine recombinante Ag28664
• Nom complet: ribonucleotide reductase M2 polypeptide
• Masse moléculaire calculée: 389 aa, 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC030154
• Symbole du gène: RRM2
• Identification du gène (NCBI): 6241
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ribonucleotide reductase M2 subunit is one of two subunits that constitute ribonucleotide reductase, the enzyme that catalyzes the conversion of ribonucleotide 5'-diphosphates into 2'-deoxyribonucleotides, a rate-limiting step in the production of 2'-deoxyribonucleoside 5'-diphosphates (dNTP) required for DNA synthesis and repair that is required for DNA synthesis and repair [PMID:20825972, 19250552]. RRM2 is only expressed during the late G1/early S phase, and degraded in late S phase, and the activity of RNR, and therefore DNA synthesis and cell proliferation, is controlled during the cell cycle by the synthesis and degradation of RRM2 subunit [PMID:3894352].

{{ptg:RelatedPrimaryAntibodies}}