Anticorps Polyclonal de lapin anti-RRP1B

RRP1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 27723-1-AP

Synonymes

RRP1-like protein B, RRP1 like protein B, Ribosomal RNA processing protein 1 homolog B

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Galerie de données de validation

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  WB analysis of HEK-293 using 27723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27723-1-AP (RRP1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27723-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27723-1-AP (RRP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27723-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27723-1-AP (RRP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 27723-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27723-1-AP (RRP1B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 27723-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27723-1-AP (RRP1B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 27723-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27723-1-AP (RRP1B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 27723-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27723-1-AP (RRP1B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 27723-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using 27723-1-AP (RRP1B antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules K-562
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

27723-1-AP cible RRP1B dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RRP1B Protéine recombinante Ag23405
• Nom complet: ribosomal RNA processing 1 homolog B (S. cerevisiae)
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC028386
• Symbole du gène: RRP1B
• Identification du gène (NCBI): 23076
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribosomal RNA Processing 1 Homolog B (RRP1B) is a protein that has been implicated in several biological processes, particularly in the context of cancer progression and viral replication. RRP1B was first identified as a metastasis susceptibility gene in breast cancer through its ability to modulate gene expression in a manner that can be used to accurately predict prognosis in breast cancer . It has been shown to interact with the metastasis modifier gene, Sipa1, and modulate elements of metastasis predictive gene expression signatures.

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