Anticorps Polyclonal de lapin anti-RRS1

• Phare
• Validé par KD/KO

RRS1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 15329-1-AP

Synonymes

KIAA0112, RRR, RRS1

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Galerie de données de validation

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  WB analysis of K-562 using 15329-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15329-1-AP (RRS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15329-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15329-1-AP (RRS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15329-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15329-1-AP (RRS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 15329-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15329-1-AP (RRS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 15329-1-AP

  IP result of anti-RRS1 (IP:15329-1-AP, 4ug; Detection:15329-1-AP 1:800) with K-562 cells lysate 3200ug.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa
• Résultats positifs en IP: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below

Informations sur le produit

15329-1-AP cible RRS1 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RRS1 Protéine recombinante Ag7030
• Nom complet: RRS1 ribosome biogenesis regulator homolog (S. cerevisiae)
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC001811
• Symbole du gène: RRS1
• Identification du gène (NCBI): 23212
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Sci Adv; Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.; Authors - Pengbo Cao ; KD Validated
human	WB	Nat Commun; Targeting dual-specificity tyrosine phosphorylation-regulated kinase 2 with a highly selective inhibitor for the treatment of prostate cancer.; Authors - Kai Yuan
human	WB	Biol Direct; Depletion of LONP2 unmasks differential requirements for peroxisomal function between cell types and in cholesterol metabolism; Authors - Akihiro Yamashita
human	WB	Tissue Cell; Silencing ribosome biogenesis regulator 1 homolog (RRS1) inhibits angiogenesis and cisplatin resistance of lung cancer cells by activating ferroptosis mediated by p53 pathway; Authors - Ling Lin
mouse	WB	Adv Sci (Weinh); m6A Reader hnRNPA2B1 Modulates Late Pachytene Progression in Male Meiosis Through Post-Transcriptional Control; Authors - Lisha Yin

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