Anticorps Polyclonal de lapin anti-RS1

RS1 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 24430-1-AP

Synonymes

XLRS1, Retinoschisin, retinoschisin 1, X linked juvenile retinoschisis protein, X-linked juvenile retinoschisis protein

---

Galerie de données de validation

• 

  WB analysis of rat retina using 24430-1-AP

  rat retina tissue were subjected to SDS PAGE followed by western blot with 24430-1-AP (RS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 24430-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 24430-1-AP (RS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse eye using 24430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 24430-1-AP (RS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 24430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 24430-1-AP (RS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 24430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 24430-1-AP (RS1 antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse eye using 24430-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using RS1 antibody (24430-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse eye using 24430-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using RS1 antibody (24430-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse eye using 24430-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse eye tissue using 24430-1-AP (RS1 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: tissu rétinien de rat, tissu oculaire de souris
• Résultats positifs en IHC: tissu oculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu oculaire de souris,
• Résultats positifs en IF-Fro: tissu oculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

24430-1-AP cible RS1 dans les applications de WB, IHC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RS1 Protéine recombinante Ag18885
• Nom complet: retinoschisin 1
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC141638
• Symbole du gène: RS1
• Identification du gène (NCBI): 6247
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RS1 is also named as XLRS1. RS1 can bind negatively charged membrane lipids, such as phosphatidylserine and phosphoinositides. RS1 is required for normal structure and function of the retina (PMID:19093009). It may play a role in cell-cell adhesion processes in the retina (PMID:27114531). RS1 is high expression in the retina (at protein level) (PMID:10915776, PMID:9326935). It is detected in the inner segment of the photoreceptors, the inner nuclear layer, the inner plexiform layer and the ganglion cell layer. At the macula, it is expressed in both the outer and inner nuclear layers and in the inner plexiform layer (PMID:10915776, PMID:10915776). And RS1 is undetectable in the inner plexiform layers and the inner nuclear layer (PMID:10915776)

Publications

Species	Application	Title
mouse	IF	Biochem Biophys Rep; Intravitreal injection of new adeno-associated viral vector: Enhancing retinoschisin 1 gene transduction in a mouse model of X-linked retinoschisis; Authors - Yan Sun
mouse	WB	Hum Gene Ther; Intravitreal Delivery of rAAV2-hSyn-hRS1 Results in Retinal Ganglion Cell-Specific Gene Expression and Retinal Improvement in the Rs1-KO Mouse; Authors - Yangyang Zheng

{{ptg:RelatedPrimaryAntibodies}}