Anticorps Polyclonal de lapin anti-RSK2

• Phare
• Validé par KD/KO

RSK2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 23762-1-PBS

Synonymes

RPS6KA3, ISPK1, ISPK 1, Insulin-stimulated protein kinase 1, HU 3

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Galerie de données de validation

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  WB analysis of K-562 using 23762-1-AP (same clone as 23762-1-PBS)

  WB result of RSK2 antibody (23762-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RSK2 transfected K-562 cells. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  WB analysis using 23762-1-AP (same clone as 23762-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 23762-1-AP (same clone as 23762-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 23762-1-AP (same clone as 23762-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 23762-1-AP (same clone as 23762-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 23762-1-AP (same clone as 23762-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 23762-1-AP (same clone as 23762-1-PBS)

  IP result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:4000) with K-562 cells lysate 3680 ug. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 23762-1-AP (same clone as 23762-1-PBS)

  IP result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:1000) with K-562 cells lysate 4000ug. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 23762-1-AP (same clone as 23762-1-PBS)

  IP result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:1000) with K-562 cells lysate 4000 ug. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 23762-1-AP (same clone as 23762-1-PBS)

  IP result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:1000) with K-562 cells lysate 4000 ug. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 23762-1-AP (same clone as 23762-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23762-1-AP (RSK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 23762-1-AP (same clone as 23762-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23762-1-AP (RSK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 23762-1-AP (same clone as 23762-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 23762-1-AP (RSK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 23762-1-AP (same clone as 23762-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23762-1-AP (RSK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 23762-1-AP (same clone as 23762-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 23762-1-AP (RSK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 23762-1-AP (same clone as 23762-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 23762-1-AP (RSK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 23762-1-AP (same clone as 23762-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 23762-1-AP (RSK2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IF Staining of HUVEC using 23762-1-AP (same clone as 23762-1-PBS)

  Immunofluorescent analysis of HUVEC cells using 23762-1-AP (RSK2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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Informations sur le produit

23762-1-PBS cible RSK2 dans les applications de WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RSK2 Protéine recombinante Ag20620
• Nom complet: ribosomal protein S6 kinase, 90kDa, polypeptide 3
• Masse moléculaire calculée: 740 aa, 84 kDa
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC096301
• Symbole du gène: RSK2
• Identification du gène (NCBI): 6197
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RPS6KA3(Ribosomal protein S6 kinase alpha-3) is also named as ISPK1, MAPKAPK1B, RSK2 and belongs to the protein kinase superfamily. It localizes at the kinetochores under spindle checkpoint conditions, and during mitosis, associated with the centrosomes, the spindle and at the mid-body(PMID:20383198). It is required for EGF activated phosphorylation of histone H3, which may cause chromatin remodeling and facilitate gene transcription and maybe playing an essential regulatory role in protein synthesis during cell proliferation.

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