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Anticorps Polyclonal de lapin anti-RSK4

RSK4 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21877-1-PBS

Synonymes

RPS6KA6, p90RSK6, pp90RSK4, S6K alpha 6

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using RSK4 Polyclonal antibody (21877-1-AP)

    WB analysis using 21877-1-AP (same clone as 21877-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21877-1-AP (RSK4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21877-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of PC-12 cells using RSK4 Polyclonal antibody (21877-1-AP)

    IP experiment of PC-12 using 21877-1-AP (same clone as 21877-1-PBS)

    IP result of anti-RSK4 (IP:21877-1-AP, 4ug; Detection:21877-1-AP 1:800) with PC-12 cells lysate 1560 ug. This data was developed using the same antibody clone with 21877-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using RSK4 Polyclonal antibody (21877-1-AP)

    IP experiment of MCF-7 using 21877-1-AP (same clone as 21877-1-PBS)

    IP result of anti-RSK4 (IP:21877-1-AP, 4ug; Detection:21877-1-AP 1:800) with MCF-7 cells lysate 1520 ug. This data was developed using the same antibody clone with 21877-1-PBS in a different storage buffer formulation.

Informations sur le produit

21877-1-PBS cible RSK4 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène RSK4 Protéine recombinante Ag16299
Nom complet ribosomal protein S6 kinase, 90kDa, polypeptide 6
Masse moléculaire calculée 745 aa, 84 kDa
Poids moléculaire observé84 kDa
Numéro d’acquisition GenBankBC148802
Symbole du gène RPS6KA6
Identification du gène (NCBI) 27330
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ribosomal s6 kinase4 (RSK4), an X-linked gene, firstly was found as to be a potential tumor suppressive gene in a variety of cancers and is widely participated in signaling pathway. RSK4 is a predominantly cytosolic protein with very low expression and several characteristics of the RSK family kinases, including the presence of two functional kinase domains and a C-terminal docking site for ERK (PMID: 39114716, 30579335). Endogenous RSK4 showed the same level of phosphorylation of Ser232, Ser372, and Ser389 in starved and growth factor-stimulated cells, and mutational analysis demonstrated that these sites are important in stimulation of kinase activity (PMID: 15632195).

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