Anticorps Polyclonal de lapin anti-RSL24D1

RSL24D1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25190-1-PBS

Synonymes

C15orf15, HRP L30 iso, L30, My024, Ribosomal L24 domain-containing protein 1

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Galerie de données de validation

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  WB analysis of HepG2 using 25190-1-AP (same clone as 25190-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25190-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 25190-1-AP (same clone as 25190-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25190-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 25190-1-AP (same clone as 25190-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25190-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 25190-1-AP (same clone as 25190-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25190-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 25190-1-AP (same clone as 25190-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RSL24D1 antibody (25190-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 25190-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25190-1-PBS cible RSL24D1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RSL24D1 Protéine recombinante Ag18195
• Nom complet: ribosomal L24 domain containing 1
• Masse moléculaire calculée: 163 aa, 20 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC008409
• Symbole du gène: RSL24D1
• Identification du gène (NCBI): 51187
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RSL24D1 (Ribosomal L24 Domain Containing 1) is a ribosomal protein that is primarily localized in the nucleolus and is involved in the biogenesis of the 60S ribosomal subunit. In mouse embryonic stem cells (ESCs), the expression level of RSL24D1 is high and significantly decreases during cell differentiation. The role of RSL24D1 in ribosome biogenesis includes facilitating proper protein docking in nucleolar and cytoplasmic precursor particles. After the completion of biogenesis, RSL24D1 dissociates from the particles and is thought to be replaced by ribosomal protein L24.

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