Anticorps Polyclonal de lapin anti-RSPH9

RSPH9 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(10)

Avis (1)

N° de cat : 23253-1-AP

Synonymes

MRPS18AL1, CILD12, C6orf206

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Galerie de données de validation

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  WB analysis of mouse testis using 23253-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 23253-1-AP (RSPH9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 23253-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23253-1-AP (RSPH9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 23253-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 23253-1-AP (RSPH9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 23253-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 23253-1-AP (RSPH9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat brain using 23253-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 23253-1-AP (RSPH9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules NIH/3T3, tissu testiculaire de rat
• Résultats positifs en IHC: tissu cérébral de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IF: See 7 publications below

Informations sur le produit

23253-1-AP cible RSPH9 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RSPH9 Protéine recombinante Ag19745
• Nom complet: radial spoke head 9 homolog (Chlamydomonas)
• Masse moléculaire calculée: 276 aa, 31 kDa
• Poids moléculaire observé: 31-35 kDa
• Numéro d’acquisition GenBank: BC029519
• Symbole du gène: RSPH9
• Identification du gène (NCBI): 221421
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RSPH9 (Radial Spoke Head 9 Homolog) is a protein involved in the structure and function of cilia and flagella. Cilia and flagella are hair-like structures that extend from the surface of many eukaryotic cells and play critical roles in cell motility, sensory perception, and signaling. The RSPH9 protein is a component of the radial spoke, a structure within the axoneme (the core of cilia and flagella) that is essential for the regulation of ciliary and flagellar movement.

Publications

Species	Application	Title
mouse	WB	Elife; LRRC23 truncation impairs radial spoke 3 head assembly and sperm motility underlying male infertility; Authors - Jae Yeon Hwang
human	IF	Hum Reprod; CALR3 defects disrupt sperm-zona pellucida binding in humans: new insights into male factor fertilization failure and relevant clinical therapeutic approaches; Authors - Yang Gao
human	WB, IF	Hum Mol Genet; Loss of DRC1 function leads to multiple morphological abnormalities of the sperm flagella and male infertility in human and mouse.; Authors - Jintao Zhang
		Eur J Hum Genet; Bi-allelic variants in human TCTE1/DRC5 cause asthenospermia and male infertility.; Authors - Shushu Zhou
mouse	WB,IF	Cell Rep; Differential requirements of IQUB for the assembly of radial spoke 1 and the motility of mouse cilia and flagella; Authors - Xin Zhang
mouse	WB	EBioMedicine; CFAP70 is a solid and valuable target for the genetic diagnosis of oligo-astheno-teratozoospermia in infertile men; Authors - Hui-Juan Jin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Célia (Verified Customer) (10-28-2024)

I tested 1/100 to be sure that it works in IF but the concentration was a little high so 1/200 should still be fine.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Spermatozoa

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