Anticorps Polyclonal de lapin anti-RSPH9
RSPH9 Polyclonal Antibody for WB, IHC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IHC, IF, ELISA
Conjugaison
Non conjugué
N° de cat : 23253-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | tissu testiculaire de souris, cellules NIH/3T3, tissu testiculaire de rat | 
| Résultats positifs en IHC | tissu cérébral de rat, il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. | 
Dilution recommandée
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 | 
| Immunohistochimie (IHC) | IHC : 1:50-1:500 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 6 publications below | 
| IF | See 7 publications below | 
Informations sur le produit
23253-1-AP cible RSPH9 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris | 
| Réactivité citée | Humain, souris | 
| Hôte / Isotype | Lapin / IgG | 
| Clonalité | Polyclonal | 
| Type | Anticorps | 
| Immunogène | RSPH9 Protéine recombinante Ag19745 | 
| Nom complet | radial spoke head 9 homolog (Chlamydomonas) | 
| Masse moléculaire calculée | 276 aa, 31 kDa | 
| Poids moléculaire observé | 31-35 kDa | 
| Numéro d’acquisition GenBank | BC029519 | 
| Symbole du gène | RSPH9 | 
| Identification du gène (NCBI) | 221421 | 
| Conjugaison | Non conjugué | 
| Forme | Liquide | 
| Méthode de purification | Purifié par affinité contre l'antigène | 
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol | 
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. | 
Informations générales
RSPH9 (Radial Spoke Head 9 Homolog) is a protein involved in the structure and function of cilia and flagella. Cilia and flagella are hair-like structures that extend from the surface of many eukaryotic cells and play critical roles in cell motility, sensory perception, and signaling. The RSPH9 protein is a component of the radial spoke, a structure within the axoneme (the core of cilia and flagella) that is essential for the regulation of ciliary and flagellar movement.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for RSPH9 antibody 23253-1-AP | Download protocol | 
| IHC protocol for RSPH9 antibody 23253-1-AP | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 
Publications
| Species | Application | Title | 
|---|---|---|
| Elife LRRC23 truncation impairs radial spoke 3 head assembly and sperm motility underlying male infertility | ||
| Hum Reprod CALR3 defects disrupt sperm-zona pellucida binding in humans: new insights into male factor fertilization failure and relevant clinical therapeutic approaches | ||
| Hum Mol Genet Loss of DRC1 function leads to multiple morphological abnormalities of the sperm flagella and male infertility in human and mouse. | ||
| Eur J Hum Genet Bi-allelic variants in human TCTE1/DRC5 cause asthenospermia and male infertility. | ||
| Cell Rep Differential requirements of IQUB for the assembly of radial spoke 1 and the motility of mouse cilia and flagella | ||
| EBioMedicine CFAP70 is a solid and valuable target for the genetic diagnosis of oligo-astheno-teratozoospermia in infertile men | 
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Célia (Verified Customer) (10-28-2024) | I tested 1/100 to be sure that it works in IF but the concentration was a little high so 1/200 should still be fine. 
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