Anticorps Polyclonal de lapin anti-RTF1

RTF1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12170-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of MCF-7 using 12170-1-AP (same clone as 12170-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12170-1-AP (RTF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 12170-1-AP (same clone as 12170-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12170-1-AP (RTF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 12170-1-AP (same clone as 12170-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12170-1-AP (RTF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 12170-1-AP (same clone as 12170-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12170-1-AP (RTF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 12170-1-AP (same clone as 12170-1-PBS)

  IP result of anti-RTF1 (IP:12170-1-AP, 4ug; Detection:12170-1-AP 1:500) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 12170-1-AP (same clone as 12170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12170-1-AP (RTF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 12170-1-AP (same clone as 12170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12170-1-AP (RTF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12170-1-PBS cible RTF1 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RTF1 Protéine recombinante Ag2812
• Nom complet: Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• Masse moléculaire calculée: 585aa,67 kDa; 710aa,80 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC015052
• Symbole du gène: RTF1
• Identification du gène (NCBI): 23168
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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