Anticorps Polyclonal de lapin anti-RTKN2

• Phare
• Validé par KD/KO

RTKN2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 17458-1-AP

Synonymes

PH domain-containing family K member 1, Pleckstrin homology domain-containing family K member 1, PLEKHK1, rhotekin 2, Rhotekin-2

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Galerie de données de validation

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  WB analysis of HL-60 using 17458-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17458-1-AP (RTKN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17458-1-AP

  WB result of RTKN2 antibody (17458-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RTKN2 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 17458-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17458-1-AP (RTKN2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 17458-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 17458-1-AP (RTKN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lymph using 17458-1-AP

  mouse lymph tissue were subjected to SDS PAGE followed by western blot with 17458-1-AP (RTKN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17458-1-AP (RTKN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17458-1-AP (RTKN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 17458-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using RTKN2 antibody (17458-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HEK-293, tissu lymphatique de souris
• Résultats positifs en IHC: tissu placentaire humain, tissu cérébral humain, tissu cutané humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

17458-1-AP cible RTKN2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RTKN2 Protéine recombinante Ag11098
• Nom complet: rhotekin 2
• Masse moléculaire calculée: 163aa,19 kDa; 609aa,69 kDa
• Poids moléculaire observé: 69 kDa
• Numéro d’acquisition GenBank: BC025765
• Symbole du gène: RTKN2
• Identification du gène (NCBI): 219790
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Sci Rep; Cellular hormetic response to 27-hydroxycholesterol promotes neuroprotection through AICD induction of MAST4 abundance and kinase activity.; Authors - Brendan Gongol ; KD Validated
human	WB,IHC	J Viral Hepat; Depletion of circ_0088046 suppressed cell growth and motility of hepatocellular carcinoma via circ_0088046-miR-1299-RTKN2 ceRNA pathway; Authors - Li Zhang
human	WB	Sci Rep; RTKN2 knockdown alleviates the malignancy of breast cancer cells by regulating the Wnt/β-catenin pathway; Authors - Xiaomei Zhang

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