Anticorps Recombinant de lapin anti-RTN2

RTN2 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250823C1

N° de cat : 86162-1-PBS

Synonymes

Neuroendocrine-specific protein-like 1, Neuroendocrine-specific protein-like I, NSPL1, NSPLI, NSP-like protein 1

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Galerie de données de validation

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  WB analysis using 86162-1-RR (same clone as 86162-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86162-1-RR (RTN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86162-1-PBS in a different storage buffer formulation.

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  WB analysis using 86162-1-RR (same clone as 86162-1-PBS)

  Various cell lysates were subjected to SDS PAGE followed by western blot with 86162-1-RR (RTN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86162-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 86162-1-RR (same clone as 86162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 86162-1-RR (RTN2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86162-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 86162-1-RR (same clone as 86162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 86162-1-RR (RTN2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86162-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02385-1

  The mean RTN2 concentration was determined to be 4623.0 ng/mL in Mouse skeletal muscle tissue extract based on a 4.1 mg/mL extract load.

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  Cytometric bead array standard curve of MP02385-1

  Cytometric bead array standard curve of MP02385-1, RTN2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86162-1-PBS. Detection antibody: 86162-2-PBS. Standard: Ag1655. Range: 0.469-60 ng/mL.

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  Affinity and Kinetic Characterization of 86162-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86162-1-RR against Human RTN2 were performed. The affinity constant is 0.358 nM.

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Informations sur le produit

86162-1-PBS cible RTN2 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RTN2 Protéine recombinante Ag1655
• Nom complet: reticulon 2
• Masse moléculaire calculée: 59 kDa, 51 kDa, 22 kDa
• Poids moléculaire observé: 20-23 kDa
• Numéro d’acquisition GenBank: BC014244
• Symbole du gène: RTN2
• Identification du gène (NCBI): 6253
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. Human RTN2 gene produces three isoforms termed RTN2-A, RTN2-B, and RTN2-C. Isoform RTN2-C has a calculated molecular weight of 22 kDa and is highly expressed in skeletal muscle.

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